Day 6: 10-10-2019
The same old days, I woke up around half past seven, had breakfast with my family prepared my lunch and left for the bus. At 8.50 am. my bus arrived and around 9.45 I arrived at the university nothing went wrong this time. My mom told me today was my uncle’s birthday, but it wasn’t, so embarrassing! Today Felix would be studying alone, so it was only Daphne and I. Yesterday I went to the museum, today the crown princes of Sweden with give an exhibition about something new placed in there, not sure if I should go there again today. There is a slight chance she won’t be there anymore when I am there and I have seen it all yesterday. Dilemma’s. Yesterday we had prepared the tubes with DNA in it, we put those in the 37 degrees oven and today we were going to add liquids and after each liquid centrifuge it so the waste would remain in the tube and we could get rid of it very easily. We did this twice with two different liquids and them washed them twice and centrifuged them twice as well. In the end we got the DNA left in the tube and put those in the fridge, so we could send them later the day to Germany. We looked at the samples of our own choice, more colonies had grown and there several colors on them, so more different bacteria had grown on the plates. The yoghurt was already growing however, tomorrow we could even see the colonies way better and more appart. Than we had two other samples one of them that grows in soil and in your stomach and another one, there you could see the difference between them as well. The one from the soil was way thicker and had curved edges, while the other was thinner and shinny and had sharp edges. Tomorrow we would be looking at them all under the microscope. We had lunch again at 12.30. After lunch we would be making a solution which Grant was using yesterday, but was almost empty. Oh yeah, we poured the DNA of the banana in a tube! Yesterday we made a new plan which was making that 500 ml tube of solution Grant didn’t want to make. So we used 90.4 grams of a solid and added water to it, we had to make the pH to 8, so we started with 4 pH and added KOH until the pH was 8. This took so long, I at least held the pH measuring for ten minutes. We finally made the pH 8 and than we left it on the machine that spins it around. We continued with the tubes we prepared yesterday. We poured 15ml in the other tubes with the barcodes on it. I kinda screwed up since I didn’t know which ones I already did. But we put them all in a bag and walked to the building where the post office is and we put them in the mail, because around 15.00 they get picked up. We had a little break after that and we would recap the liquid we were trying to make with the pH of 8. So we were including more solids in the bottle and that made the Buffer done. We used 40 ml of the Buffer put it into a larger cane from 1 liter and added the rest with water. Than the Buffer part was over as well. We had done everything so in theory we were done for the day, but I asked if we could do the ice experiment so we did. We got dried ice from the basement, it was really cold. We prepared a glass with water and tried to cut the ice in pieces, it was harder than we thought it would be. Daphne used a knife for it. I poured ice in the water and it was amazing to see what the reaction was. Later on we used another bottle filled with ethanol and did the same, poured ice in that as well. After a while the ethanol bottle was completely frozen. It was very cold when you touched it. We watched the reaction for at least 15 min, but after that it was enough so we cleaned it up and went to the tunnelbana. Daphne joined me, because she had to go to Tekniska Högskolan to her Danish friend. It was so crowded in the train, when Daphne left there were many people there, luckily I was at the end better than in the middle, because than I had no place to stay. Now comes the disgusting part, there was a man in front of me who had an disgusting smell, yak. It was so hard not to smell it, I almost threw up. But the problem was I had no other place to stay so I had pray for him to leave the next stop, but he didn’t so I had to smell that for a longer time. Finally he left, but a man who was standing behind me came in front of me who stank as well, so my luck was already over, luckily we got to the central sooner than I had hoped for it. So I went to my next train where I had a place to sit, I couldn’t stand anymore my legs were too tired. I sat down watched Full House and before I knew it I got to Akalla. I took the bus back and walked the last part home, but it was already dark believe it or not it was only 18.00 o’clock. I got home around 18.15 and changed and wanted to eat dinner, I was really hungry. But my grandfather had a new phone, the Iphone XS!!!! I had to help him with everything, before I was actually eating my dinner it was 19.00 o’clock, but during my dinner he came over and over and over, so at 20.00 o’clock I was really done eating. At this time my uncle came home as well, he went to the doctor and did some grocery shopping. My uncle and my grandfather went to a restaurant today. I wasn’t able to join them, because I went to the laboratorium. I did some homework, watched Netflix, at 22.00 I went to sleep again.
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